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The new physical insight of the present studies is that 1 acts as excellent discriminator molecule for C70 in presence of AgNp as selectivity in binding is estimated to be ∼21.7 in presence of AgNp compared to the situation when fullerene 1 mixture does not contain any AgNp (i.e., selectivity in binding = ∼5.0) in solution.
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The average lifetimes τ2 of type II binding and τ3 of type III binding were estimated to be 0.19 ± 0.01 and 0.85 ± 0.08 s, respectively.
By comparing the dye's fluorescence intensities at zero and saturating (i.e., 40 ng/μL) λDNA concentrations, the fluorescence enhancement of the dye upon DNA binding was estimated to be approximately 70 times.
Co II) confirmed the presence of a tetrahedral site, and on the basis of the competition, the dissociation constant for Zn II) binding was estimated to be on the order of 10 9 M.
The adjusted IC50 for protein binding was estimated to range from 8to2020 ng/mL against reference viral strains with a conventional cycle cell infection and the PhenoSenseTM single assay (ViroLogic, Inc, South San Francisco, CA, USA), respectively.
In the minibody reported by Pessi et al., a 61-residue all β-sheet structure, the dissociation constant for Zn II) binding was estimated to have a lower limit of ∼10 6 M. While this represents fairly strong binding, it is relatively weak when compared to that of native zinc enzymes that generally have dissociation constants in the nanomolar to picomolar range.
The most fascinating feature of the present study is that 1 binds selectively C60 compared to C70 as obtained from binding constant (K) data of C60-1 (KC70-1) and C70-1 (KC70-1) complexes which are enumerated to be 265,000 dm3 mol−1 and 63,430 dm3 mol−1, respectively, and selectivity in binding (KC60-1/KC70-1) is estomated to be 4.18 as obtained from UV Vis study.
The estimated rate parameters are 0.02 binding sites originating per kb and 1 Mio years, the half-life for a BAS1-binding site is estimated to be 22.7 Mio years.
Thus the differential-binding energy is estimated to be in the order of RTln(1000) (or ~7RT), so may the elastic conformational energy ΔGE be.
The resulting binding causes a conformational change that propagates to EF1, thus enhancing its Ca2+ binding, in which c12 is estimated to be ~ 1,500.
For example, DNA binding by 4-ABP is estimated to vary between individuals by as much as one millionfold between the most and least susceptible individuals [ 13].
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