Specific binding was established by coincubating with 10-, 25- and 50-fold excess of unlabelled oligonucleotide.
In this and subsequent experiments, specificity of binding was established by competition with free 20(S -OHC.
Streptavidin-coated donor beads were used in conjunction with glutathione-coated acceptor beads, and the binding was established by monitoring proximity-based luminescent signal.
The strength and mode of DNA binding was established by thermal melting stabilization, direct titration and viscometric studies of host duplex length.
For naïve T cells, subsequent firm binding was established by ICAM-1 and ICAM-2; however, for cancer cells, this binding could not be blocked by anti-ICAM-1 and ICAM-2 antibodies.
Local and remote bindings are established by creating new subscription relationships, supporting one-to-one, many-to-one, and one-to-many bindings (as specified in [13]).
Each time a form creates a lexical binding of a variable, a fresh binding is established.
Hanson MA, Cherezov V, Griffith MT, Roth CB, Jaakola VP, Chien EY, et al. A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor.
The ligand-binding site is established by the interface of both lobes [ 6].
Generic spinal motor neuron identity is established by cooperative binding of programming transcription factors (TFs), Isl1 and Lhx3, to motor-neuron-specific enhancers.
