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(c) FOSL1 binding is enriched in the chromatin regions with increased interactions in stimulated CD4+ T cells, while no enrichment is detected in resting CD4+ T cells.
We found that functional TF binding is enriched in regulatory elements that harbor a large number of TF binding sites, at sites with predicted higher binding affinity, and at sites that are enriched in genomic regions annotated as "active enhancers". Chromatin architectural proteins interact with nucleosomes to modulate chromatin accessibility and higher-order chromatin structure.
This is supported by the fact that 'metal-ion binding' is enriched in the core, as many metabolic enzymes have metal-binding catalytic centres.
By high-resolution mapping of intra- and interchromosome interactions, Rickman et al. [ 80] demonstrated that ERG binding is enriched in hotspots of differential chromatin interaction.
For most ChIP-seq datasets, binding is enriched in the vicinity of the breakpoints near genes and enriched even more (p = 2.28⋅10-37) around breakpoints far from genes.
In Cusanovich et al.'s paper [ 29], they reported that functional TF binding is enriched in the regulatory regions with a larger number of bound TFs and more binding sites.
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The GO term 'lipid binding' was enriched in both WD treatments.
Finally, among Molecular Function terms, 'nucleotide binding'hydrolaseactivityvity' and 'RNA binding' were enriched in non-conserved gene-like sequences.
Our results showed that genes related to "purine nucleotide binding" and "nucleotide binding" were enriched in the targets of up-regulated miRNAs.
Categories "trans-membrane transporter activity", "substrate-specific transport activity" and to lesser extent "transferase-activity" and "nucleotide binding" are enriched in glumes.
Moreover, we found that the GO terms "hydrolase activity, acting on acid anhydrides", "tetrapyrrole binding", "iron ion binding" and "heme binding" were enriched in the "molecular function" category (Additional files 11 and 14).
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