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While BES1 itself binds to the At4g18010 promoter (P3) in WT plants, such binding is enhanced in RD26OX plants (Fig. 4d, columns 3 and 4), suggesting that BES1 and RD26 together enhance binding to the promoter region.
In the case of the TnH peptides, there is clear evidence that Ca2+ binding is enhanced in the presence of the peptides.
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In both cases, binding was enhanced in the presence of the unique site.
We found that the SOST promoter contains a conserved Smad binding site in the CpG island located upstream of the TSS and that Smad1/5/8 binding was enhanced in OA compared to normal chondrocytes (p = 0.05) and in BMP-2-treated OA compared to untreated chondrocytes (p = 0.05) (Fig. 5a and c).
The dissociation of 3 nM BODIPY-TMR-CGP binding was enhanced in the presence of 1 µM CGP 12177 and 1 µM propranolol with dissociation rates of 0.186 ± 0.008 (n = 6) and 0.189 ± 0.007 min−1 (n = 6), respectively (Fig. 9 A ).
At the ADH2 promoter, Cat8-binding is enhanced in the presence of Adr1, but the reverse is not true [9].
In HD, the binding is enhanced thereby displacing dynamin.
The data reported by Strutt and Strutt [7] agree with Wu and Mlodzik [8], providing independent evidence from tissue culture experiments that Vang and Fz can bind in trans, a binding that is enhanced in the presence of Fmi.
Electrophoretic mobility shift assays (EMSA) and ChIP assays demonstrated that both Sp1 and Sp3 bind to these elements and the binding activity is enhanced in senescent cells.
Others have shown that USF binding activity is enhanced in response to LPS [ 56].
In contrast, at 1 s the 354-nt RRE shows overall higher normalized SHAPE changes than all three mutants, indicating that Rev binding rate is enhanced in the context of the full-length RRE with intact Stem I region.
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