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The specificity of the [35S]GTPγS binding is demonstrated in Fig. 7 (C, F, I) where an excess of cold GTPγS was included in the incubation.
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Not only can binding be demonstrated in vitro, the two proteins also co-localize in activated cells at the cortex.
Specific binding of 111In-DTPA-cNGR was confirmed in the Matrigel model and, moreover, binding was demonstrated in the infarcted myocardium and infarct border zone.
Estradiol binding was demonstrated in MCF-10A cell membrane fractions and may explain the estradiol action in these cells that lack intracellular ER.
No binding was demonstrated in CAIX negative CaKi 2 and HUVEC cells.
Significant glucose-fiber binding was demonstrated in the in-vitro models.
Increased C-DED binding was demonstrated in the brain of patients with Creutzfeldt-Jacob disease [ 67].
Agonist induced changes in GTPγS binding were demonstrated in fused Gsα/β2-adrenoceptors where agonist efficacy was well reflected by changes in the kinetics of GTPγS binding (Wenzel-Seifert and Seifert, 2000; Seifert et al., 2001).
Specific low affinity (Kd = 166 nM), high capacity (Bmax = 1.2 pmol mg-1 protein) somatostatin binding was demonstrated in 22 of the gastric cancers and 17 of the colorectal cancers (Kd = 140 nM, Bmax = 1.8 pmol mg-1 protein).
The lack of involvement of the BP in copper binding is demonstrated by IR spectroscopy.
A functional role for the cyclic AMP response element-binding protein (CREB) is demonstrated in controlling gene expression in hypoxia.
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