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The lack of involvement of the BP in copper binding is demonstrated by IR spectroscopy.
The importance of KLHL3 Asn for WNK4 binding is demonstrated by the decreased affinity of the WNK4 19-residue peptide for KLHL3 N529K relative to wild-type KLHL3.
That the fast phase represents the Ca2+ binding is demonstrated by the observation that mixing of isolated cTn and cTn incorporated into myofibrils with high [Ca2+] induces the fast phase, which increases the fluorescence by ∼60% within the dead time of the stopped-flow apparatus (2.2 ms) (Fig. 2, A and E ).
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Selective binding was demonstrated by turbidity assays with Ricinus Communis Agglutinin (RCA120) lectin.
The response of aptamer upon target binding was demonstrated by quartz crystal microbalance (QCM) method and the microstructure of hydrogel was characterized by SEM.
Specific aptamer-STIV binding was demonstrated by EMSA assays.
These expression patterns were validated by Quantitative Real Time PCR (Q-RT-PCR) and NF-κB binding was demonstrated by chromatin immunoprecipitation (ChIP) assays.
The specificity of Zur binding was demonstrated by absence of shift when the probe containing the coding region of the stationary phase-induced gene cspD was used, even at the highest Zur concentration (1 μM).
Specific binding was demonstrated by using a Toscana virus, dengue viruses, West Nile virus, and St . Louisencephalitis virus mouse hyperimmune ascitic fluid virus (11 ) and a goat anti-mouse peroxidase-labeled conjugate (Interchim).
However, we could not find TF binding sites upstream of several genes that lack σ-dependent promoters, despite the fact that the binding was demonstrated by DAP-chip (for example, DVU3131 for DVU0804 protein, DVU0736 for DVU0744 protein and DVU0328, DVU0330, DVU1639, DVU2842, DVU1017 for DVUA0057 protein).
For IgG detection, antibodies to Toscana virus, dengue viruses, West Nile virus, and St . Louisencephalitis virus were captured by goat anti-mouse IgG antibodies; viral antigens were followed by test sera; and specific binding was demonstrated by using a peroxidase-labeled goat anti-human IgG conjugate.
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