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Colloidal noble metal nanoparticles, in particular, have found application in a variety of assay formats in which analyte binding is coupled to particle adsorption.
In addition we ask the question: What if data cannot be fit using the fitting functions incorporated in the data-analysis software that came with your ITC? Examples where such data might be generated include systems following non 1 n binding patterns and systems where binding is coupled to other events such as ligand dissociation.
However, the binding enthalpy can become exothermic (ΔH<0) if metal binding is coupled to a protein conformational change [39], [40].
They may impair the mechanism by which nucleotide binding is coupled to channel gating.
The precursor binding is coupled to the Mia40-induced folding of the first substrate helix, creating a partially folded ITS.
Such a structure would also provide key insights into how ATP binding is coupled to both neck linker docking and force generation.
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Thus, we present four DMO structures with resolutions ranging from 1.95 to 2.2 Å, which, in sum, provide a snapshot of a dynamic enzyme where metal binding and substrate binding are coupled to observed structural changes in the non-heme iron and catalytic sites.
At the same time, the active site rearrangements induced by Mg2+-ATP binding are coupled to the distortion of the seven-stranded β-sheet forcing the upper 50 kDa subdomain to undergo a large movement, which reduces the contact area and weakens the affinity to actin.
In a processive mode of enzymatic catalysis, a single substrate binding event is coupled to multiple rounds of catalytic turnover.
Thus, the innovation of metazoan catenins with PDZ binding motifs is coupled to the appearance in the most basal metazoa of PDZ domains, both in the form of novel proteins, and in new or altered PDZ domains within existing proteins.
Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction.
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