We observed a phenomenon that these phages displayed a common motif but showed different binding ability further suggests that the motif is important for the binding while this binding ability is coordinated by flanking sequences.
In a different computational study, a different binding site for Na3 was proposed on the basis of grand canonical Monte Carlo and MD simulations, which is ∼8 Å from our proposed Na3 binding site and is coordinated by Thr314, Ala353, and Asn401 as well as the bound substrate.
Additionally, combination peaks at (νdq, ν+ + ν−) ≈ (4, 2.5) MHz and (νdq, 2ν+) ≈ (4, 3.2) MHz were present (Figure 4c,d), similar to those observed for other Cu2+ binding proteins where the metal is coordinated by two His residues [47].
Despite this difference, its binding to the active sites is coordinated by three conserved histidine residues, [ 36].
This cycle is coordinated by binding and breaking down a molecule called ATP, which also provides the energy needed to take the next step.
High-resolution crystallographic studies of the engineered Zn2+-binding MBP molecule demonstrate that Zn2+ is coordinated by residues on the N-terminal lobe only, and therefore Zn2+ binding does not require the protein to adopt a fully closed conformation.
This alternating cycle is coordinated by kinesin binding to and breaking down a molecule called ATP to form another molecule called ADP, which releases the energy needed for its next step.
The second NEAT domain of IsdB is structurally similar to other heme binding NEAT domains, and heme-iron is coordinated by a tyrosine residue as predicted; however, we have also shown that Met362 coordinates the heme-iron on the distal side, resulting in an unprecedented mode of heme-iron coordination.
The carbohydrate binding Ca2+ ion, conserved in all structures of CLDs, is coordinated by amino acids: Gln88, Asp90, Glu97, Asn111, and Asp112 and by a water molecule.
Gene expression is coordinated by complex regulatory mechanisms that involve transcription factor binding and chromatin modification including DNA methylation.
