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Compound binding is captured in complex with representative LpxC orthologs, and structural analysis reveals large conformational differences that mostly reflect inherent molecular features of distinct LpxC orthologs, whereas ligand-induced structural adaptations occur at a smaller scale.
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This is the case of the well known nucleotide-binding region between residues 10 17 (GAGGVGKS), which is captured in the homogeneous cluster F.c.37.1.3.11.398 (Figure 2D and 2E), having 76 fragments from 75 'P-loop 'P-looping nucontainingriphosphate hydrolases' proteinucleotide
Given these complexities, it seems unlikely that the influence of DNA methylation on protein DNA binding can be captured in a small set of general rules.
Interdependencies between distinct binding events are captured in the optional 'attributeList' element within the 'interaction' element, which allows for additional description of the interaction data in a semi-structured manner.
We also treated the lysates with 60 μg of RNase A or 50 unit of the benzonase (Sigma) to avoid nonspecific RNA binding proteins that might be captured in FBP1 immunoprecipitation (FBP-IP) via RNA bridging.
In addition, the initial binding process of a soluble ligand to the substrate-binding site of SULT was captured in unbiased 100 ns simulations using the software Desmond [3].
These resulting two nucleotides could be captured in tube and inhibited binding by primer, thus resulting in an absence of amplification product.
It is well known that substrate binding may induce so-called occluded conformations, which have been captured in a number of reported crystal structures (Deng et al. 2015).
Histamine-binding proteins may only be expressed during the later phases of feeding as no sequences were captured in the unfed library and only one in IR24H.
The reference surface should ideally be captured in the same manner as the target protein but in an inactive state of ligand binding.
She was captured in 2006.
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