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In addition, we show that angiocidin binds to ubiquitinated proteins on the endothelial cell surface and that this binding is blocked with antiubiquitin antibody.
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To demonstrate that the nanoworms bind to the same molecular target as Rituximab, binding was blocked with unlabeled Rituximab).
Sections were permeabilized with PBS/0.2% Tween, and unspecific antibody binding was blocked with 5% horse serum.
Non-specific binding was blocked with 5% Milk-TBS for 1 hour at room temperature.
Non-specific binding was blocked with 5 10% goat serum for 60 minutes.
Antibody non-specific binding was blocked with 3% BSA in PBS for 30 min at 22°C.
Nonspecific binding was blocked with 10% horse serum in 3% BSA.
Nonspecific binding was blocked with Fc receptor blocking agents and stained with fluorophore conjugated mAbs as previously described [10].
Slides were fixed with cold acetone and non-specific binding was blocked with image-iT FX signal enhancer (Invitrogen).
Nonspecific binding was blocked with 10% nonimmune serum of a species from which the secondary antibody had been obtained.
Non-specific antibody binding was blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature.
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