Sentence examples for binding is blocked at from inspiring English sources

It is also possible that AP1 binding is blocked at the level of several cellular promoter regions of genes involved in cell proliferation, enhancing the antitumoral capabilities of GAG-hed.

Nonspecific binding is blocked by a buffer.

However, the narrow side channel that leads from the cytoplasmic surface to the ion-binding site is blocked at pH4 by side chain rearrangements, as stated in the revised manuscript.

Cells were fixed in 4% paraformaldehyde (PFA) for 15 min and permeabilised with 0.1% Triton for 15 min at room temperature, nonspecific binding was blocked for at least 30 min using blocking buffer (PBS, 50 mM glycine, 2% bovine serum albumin (BSA), 0.01% sodium azide).

Non-specific binding was blocked for 30 min at 37°C with 5% powdered milk in 0.2% NP40 buffer.

Nonspecific binding was blocked for 1 hour at room temperature using 2% BSA (Sigma), 2% normal non-immune donkey serum (Vector Labs), and 0.05% Tween-20 in 1×PBS.

Nonspecific binding was blocked for 2 h at room temperature with nonfat milk (5 %) in TBST buffer (50mMTris-HCl, pH 7.4, 150 mM NaCl, 0.1Tween 20.1Tween

As a control to demonstrate antibody recognition of subsurface proteins, spirochetes were permeabilized by fixation with 1 ml of 100% cold methanol and incubation at −20°C for 20 min. Non-specific binding was blocked by incubation of slides at 30°C for 90 min in blocking buffer (Difco Leptospira Enrichment EMJH, BD, Sparks, MD).

Nonspecific binding was blocked using Odyssey blocking solution at room temperature (LI-COR).

Non-specific binding was blocked in normal rabbit serum at a dilution 1 : 20 for 30 min (DAKO, X0902).

Cells were washed with PBS and nonspecific antibody binding was blocked with 5% FBS at room temperature (RT) for 30 min.