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In the mature CL no IgM binding is apparent to either granulosa lutein or endothelial cells.
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This binding pocket is flanked by several polar residues (Fig. 1) whose contribution to bacterial ligand binding is apparent and variable (Virji et al., 1999; 2000).
The conversion of the dissociation constant Kd to free- energy of binding is apparent from equation ΔGo = RTlnKd [37].
The differences in toxicity are essentially ascribable to distribution, although a small effect of the receptor binding is apparent.
In the intermediate layers, a similar increase of IgM binding is apparent toward the im/s interface (#2).
As shown in Figure 3, significant binding is apparent from the detection of faster sedimenting components at ∼7 8 S at pM concentrations of EGFP.
42 The long chain requirement for Dectin-1 binding is apparent also with on-array inhibition experiments in which we evaluated activities of β1 3-linked 7, 11, and 13 mers in solution as inhibitors of Dectin-1 binding.
First, a high affinity binding was apparent that fit the classic one-site competition model expected for displacement from a single class of receptors.
Concomitant with C/EBPβ up-regulation by differentiation cocktail, a trend of increased binding was apparent in the DMI condition.
Another important observation can be made: in this experiment two cell cycles occurred and two cycles of APC/C binding were apparent.
As poly-dT is titrated in a solution of SSB (0.125 μM tetramer) in low salt conditions an initial binding event is apparent followed by a second binding event at higher poly-dT concentrations (Fig. 4C).
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