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Mrs1 binding is also required for splicing of a second group I intron, the aI5β intron from the COX1 pre-mRNA in yeast mitochondria [21].
Acetyl-lysine binding is also required for Brd2 and Brd4 to both interact with and mediate the attachment of episomal viral genomes to mitotic chromosomes [17], [18], [19], [20], [21], [22].
Again, these data equally support the "conformation cycling" model, as here two-site binding is also required for Nrf2 ubiquitination, so mutation of either binding motif will inhibit Nrf2 ubiquitination.
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Importantly, the 130 N-terminal residues in TRIAD1, that are essential for NEDD8-CUL5 RBX2 binding, were also required for enhanced activity in the presence of NEDD8-CUL5 RBX2. NEDD8-CUL5 RBX2
Receptor tyrosine phosphatases LAR, the NGL-3 binding partner, is also required for maintaining the number of excitatory synapses and dendritic spines, the expression of surface AMPARs, and the targeting of the cadherin-β-catenin complex (Dunah et al., 2005).
Although the TSG101-binding PTAP motif plays the dominant part in HIV-1 release, the ALIX-binding site is also required for optimal virus replication59.
Although the most widely studied Ras-regulated signaling pathway is the Raf/mitogen-activated protein kinase cascade, previous research in model systems has revealed that the Rac1 GTP-binding protein is also required for Ras-induced biological responses.
Last, we could show that besides hemimethylated DNA binding by the SRA domain, UHRF1 PHD binding to H3R2 is also required for H3 ubiquitination and subsequent DNA methylation.
In the future, it will be important to examine the function of spine synapses induced by the expression of NLG-1 alone, and to determine whether overexpression of its binding partner neurexin is also required.
Another strain expressing the protein missing the ARID AT-rich DNA binding domain (which is also required for demethylase function) shows a mammary phenotype.
Our results provide an explanation for this, in that although the isolated Mid domain is sufficient for LSm1 binding, Pat-C is also required in the context of full-length Pat proteins and may provide a low-affinity LSm1-binding site.
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