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In order to assess whether EG binds to dsDNA with any base preference and whether its DNA binding is affected by salts, the dye was titrated with the double-stranded AT-rich λDNA and the double-stranded GC-rich Tbr DNA, respectively, in Tris buffers with or without an inorganic salt.
To further experimentally validate whether miR-371a-5p miR-371a-5p miR-371a-5p′-UTR of BAG3 andirectlyte whether this bindsng is affectod by the g2252c nucleotide change, we performed dual-luciferase reporter assays using pMIR-reporters with either the wild-type (wt) or polymorphic putative miRNA target sites cloned downstream of the firefly luciferase gene (luc2).
Consequently, to elucidate the pathogenesis of TPI deficiency, it is of enormous importance to identify cellular factors that interact with TPI, to investigate whether their association has an effect on the catalytic activity of TPI, and obviously whether the binding is affected by the various mutations.
Furthermore, the apparent K d L-ser was ~5 times higher for this mutant indicating that serine binding is affected by this mutation (Table 2).
Hmo1 was chosen because this HMG protein regulates TORC1-dependent gene transcription [ 49- 51] and its chromatin binding is affected by TORC1-regulated histone acetylation [ 36].
The degree to which ConA's binding is affected by PEGylation is likely to be dependent on the chain length and the number of chains grafted.
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Neither affinity nor extent of [125I]Calmodulin binding was affected by l-arginine, NADPH or Tetrahydrobiopterin. Native Calmodulin and engineered Calmodulin homologs [i.e., duplicated N-terminal (CaMNN)] potently displaced [125I]Calmodulin.
Figure 3 also shows that neither dORC5 or dCDC45 binding were affected by depletion of any of the MCM proteins.
We next considered whether basal levels of NF κB DNA binding were affected by changes in p53 or MMR status.
Groups of genes involved in xenobiotic metabolism, steroid biosynthesis, and CCAAT element binding were affected by 5-aza-dC (some exclusively with 5-aza-dC and TSA).
We observed that modification of actin slightly affected parameters of TMs binding to actin alone, but only short TM isoforms binding was affected by actin truncation when the filament was activated by rigor-bound myosin heads.
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