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However, the crystal structures of OcDH revealed that the conformational change observed by L-arginine binding is a prerequisite to form the pyruvate binding site [14].
Additional studies have demonstrated that Ro60 binding is a prerequisite for the export of Y RNAs from the nucleus to the cytoplasm [21], [22].
It seems plausible that stabilization of cohesin's DNA binding is a prerequisite for sister chromatid cohesion.
Because substrate binding is a prerequisite for substrate ubiquitination, this binding selectivity of Pellinos is likely responsible, at least in part, for their different cellular functions.
DNA binding is a prerequisite for transcriptional activation, so first we investigated the ability of AcNgn3KO to bind to E box DNA by electrophoretic mobility shift assay (EMSA) (Fig. 6).
Since substrate binding is a prerequisite for the 3'-5' DNA helicase activity of RECQL4, we next examined whether the mutant proteins were able to unwind a short DNA fork substrate.
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Thus, calcium binding is a critical prerequisite for proper substrate binding.
As calcium binding is a known prerequisite for binding, as the protein conformation is to be approximated from analogous structures, and as the protein−W7 contacts can be precisely and accurately known (from experimental data presented here), a structure determination blending these different data must correctly weight their relative information contents.
The long-range electrochemical conductivity in combination with the biotin binding capability is a prerequisite for the application of the conjugates in future bio-electrochemical devices.
In some cases, modification of a binding site is a prerequisite for an interaction, while in other cases modification can further enhance the strength of an interaction (61 63).
Among other aspects, knowledge of the elementary functional units – transcription factor binding sites – is a prerequisite to understanding regulation of individual genes and their embedding into regulatory networks.
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