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Nonetheless, the single largest category of binding intervals we identified were those commonly bound by Dichaete and SoxN in both species (7415 intervals).
The core Dichaete binding intervals we identified are enriched for Sox binding motifs but we also found significant overrepresentation of binding motifs for Vfl, the GAGA-binding factor Trl and the JAK-STAT pathway transcription factor Stat92E.
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While Dichaete and SoxN commonly bind the majority of conserved binding intervals, we also identified intervals that are uniquely bound by each TF in both species.
In order to examine the patterns of motif conservation in Dichaete binding intervals, we first identified all matches to the best de novo Sox motif discovered in each set of intervals [ 90].
The QTL intervals we identified did not contain any pigmentation genes previously identified by mutagenesis studies in other species.
Within this interval, we identified a direct TTX-1-binding site with the core-binding residues located between position +176 and +179 (Procko et al. 2011).
Focusing on Dichaete binding intervals, we compared intervals that are bound in all four species, including D. pseudoobscura, to those that are unique to D. melanogaster.
For the study interval, we identified 336 patients and 373 hospitalizations.
Approximately 3000 Dichaete binding intervals were identified in D. pseudoobscura, reflecting the fact that the binding profiles were noisier in this species compared to the other three species, with less reproducible biological replicates.
A similar analysis with the BDTNP and modENCODE binding intervals again identified strong enrichment for Vfl motifs (E-scores of 9.4 and 8.1 respectively) and weaker enrichment for Sox-like motifs (E-scores 2.6 and 2.8).
The binding sites we identified correlate quite well with those identified by another group that used ChIP-seq to uncover HSF binding sites in S2 cells [31].
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