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As for the folding score test set, 100 structures (25 from each major SCOP Class) were selected, each a centroid of a cluster of binding interfaces at the 3 Å-75% level of PepX.
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Crystal structures (Elrod-Erickson et al., 1998) show side chains at pos. −1 are partially solvated, while the binding interfaces at pos. +3 and +6 are desolvated, relative to WT (see shaded and open triangles).
Although some of these sites are located in protein regions with clear functional characterization (e.g., at the nucleic acid binding interface, at intracellular trafficking signals), the significance of other selected residues remains elusive.
As described for protein L, this is ideal for transmitting mechanical signals to the binding interface at low force.
Hence, the full-length EcoRII contains three putative DNA binding interfaces: one at the C-terminal domain dimer and two at each of the N-terminal domains.
In nature, however, it is possible that a multi-interface hub transiently interacts with its individual partner through the corresponding binding interface at different spatial-temporal conditions.
Trp is also enriched at protein/protein binding interfaces of the small envelope protein of the hepatitis B virus [55] and of membrane proteins in general [56].
We identified the residues at the binding interface by plotting the combined chemical shift changes of HN and N versus the residue number and mapping them onto the structure of PHD3.
The third binding interface involves residues at the extreme amino terminal of the Vpx N-terminal extended arm that inserts into a cavity between WD40 repeats 1 and 2 on the underside of DCAF1.
The slowdown of the picosecond components reflects more rigid water networks at the binding interface and in the active site.
(A ) Overview of emetine at the binding interface formed by the three conserved rRNA helices and uS11.
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