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The biochemical composition of the binding interface was calculated using the SOLV function in the program XSAE (from Dr. C. Broger, Hoffmann-La Roche, Basel, Switzerland).
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At every rotation the electrostatic energy of the interface was calculated using COILCHECK+.
Properties of each interface were calculated using ProtorP [54] and ProFace [55].
The r2 and D' values used in Ensembl web interface are calculated separately on demand [ 23].
The local peak shear rate at the near and far blood-wall interfaces was calculated.
Cell surface areas and junctional interfaces were calculated using a built-in micrometer.
For a better resolution, a close-up view of the binding interface was developed.
An identical structural homology at the binding interface was predicted (Fig. 4A and Fig.4B).
The SGT1 HSP90 binding interface was confirmed by searching for mutants that showed specific charge compensation effects.
The RNA binding interface is also extremely diverse for the four Brix proteins.
In (C) and (D) the amino acids forming the HBGA binding interface are in cyan.
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