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Identification of ligand-protein binding interactions is a critical step in drug discovery.
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This ligand-binding interaction is a conserved feature of the mammalian Cys-loop receptors studied to-date and is found here for the second time in a distantly related nematode Cys-loop receptor.
One well-documented effect of these p300 ligand binding interactions is to facilitate the formation of a transcriptionally activating complex.
Characterization of protein PIPn binding interactions is hindered by the complexity of the membrane environment and of the PIPn structures.
(4) Finally, a variety of additional proteins have been shown to bind the C-terminus of Mcm7, but currently the physiological significance of the observed binding interactions is unknown or poorly understood.
Landscapes for a pair of sites with cooperative binding interactions are of a similar kind as shown in fig. 2(a),2(b),2(c),2(d).
The EMS studies presented above demonstrate that while IHF binds to the minimal I1 duplex to yield a distinct (specific) retarded band, nonspecific binding interactions are also observed in a similar concentration range (compare panels A and B of Figure 3).
The substrate-enzyme binding interactions are rather complex and consist of a combination of electrostatic interactions, hydrogen bonds, hydrophobic interactions, and other contributions.
However, only when the 3D geometries of their interaction patterns match can a pharmacophore (the complementary set of binding interactions) be inferred representing the possible shared binding site of the target protein.
Selectin binding interactions are calcium dependent and are dissociated in the presence of EDTA, a chelator of calcium ions.
Cell lysates were treated with or without RNase A before the immunoprecipitation to determine whether any binding interactions were dependent on association with mRNA, and the immunoprecipitated proteins were then immunoblotted with antibody to TDP-43.
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