Substantial differences in protein profiles, abundance, and IgE-binding intensity with different combinations of pH and ionic strength were found.
We then asked whether TF binding intensity also correlated with the probability that TF binding was shared in closely related mammals.
These are counted into sliding windows with csaw, to quantify binding intensity across the genome (Lun & Smyth, 2014).
Normal mammary epithelium, preneoplastic mammary lesions designated D2HAN (D2-type hyperplastic alveolar nodules) and a series of nine spontaneous tumours (D2ST1, D2ST2, D2ST3, D2ST4, D2A1, D2F2, D2.0R, D2.1, EMT6R08) derived from mice bearing D2HAN were grown in culture and analysed by flow cytometry with respect to PNA binding intensity to the cell surface.
As previously reported [ 9], we observed that the binding intensity of CTCF is negatively correlated with the methylation level at the 12th position of the binding sites (Additional file 13: Figure S8).
a, c, e The consensus binding sequences according to the binding intensities observed on the Q9 protein-binding microarray.
For IgM in particular, the binding intensities to the LPS region below 25 kDa corresponded with intensities of a smear in the higher molecular weight range.
The specific binding intensity was calculated by subtraction of the mean fluorescence intensity of the background binding from the mean fluorescence intensity of the aptamers.
The binding intensity of SSc AFAs correlated strongly with reactivity against topoisomerase I on immunoblots of fibroblast extracts, and with the immunofluorescent pattern typical of anti-topo I on permeabilized cells.
