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The specific binding intensity was calculated by subtraction of the mean fluorescence intensity of the background binding from the mean fluorescence intensity of the aptamers.
Color was developed by TMB/E substrate and the binding intensity was calculated using absorption at 450/650 nm.
For each line drawn, the protein binding intensity was calculated as the result of the difference (Max - Bkg), where Max denotes the maximal brightness across the line.
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Finally, the Relative Intensity was calculated, dividing the absolute intensity of each band by the absolute intensity of the standards.
Intensity was calculated using image J software.
The mean fluorescence intensity was calculated.
Staining intensity was calculated by colour deconvolution.
Dye intensity was calculated as 100 − lightness.
The tumor staining intensity was calculated using following formula; tumor intensity = (post-injection tumor intensity) – (pre-injection tumor intensity).
The mean fluorescent intensity was calculated using FlowJo software.
The fluorescence intensity was calculated using the ImageJ software.
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