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Normal mammary epithelium, preneoplastic mammary lesions designated D2HAN (D2-type hyperplastic alveolar nodules) and a series of nine spontaneous tumours (D2ST1, D2ST2, D2ST3, D2ST4, D2A1, D2F2, D2.0R, D2.1, EMT6R08) derived from mice bearing D2HAN were grown in culture and analysed by flow cytometry with respect to PNA binding intensity to the cell surface.
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For IgM in particular, the binding intensities to the LPS region below 25 kDa corresponded with intensities of a smear in the higher molecular weight range.
A majority of the sera had markedly increased IgE-binding intensity to the 37 kDa protein in the cooked clam extract suggesting that some new epitopes might have been exposed for IgE-binding [ 19– 22].
In contrast, glycoproteins obtained from treated cells showed high binding intensity to 10 of the 38 lectins on the array with a significant difference of p < 0.01 above the background while binding to four additional lectins showed lower intensity that was nonetheless significantly above background levels (p < 0.05).
The technique allows for the parallel interrogation of thousands of single feature polymorphisms (SFPs), i.e. differences in the binding intensity to a particular oligonucleotide probe between two different samples of genomic DNA.
After 4 rounds of sorting the binding intensity to CD64 (Fig. 3A) and anti-CH2 (Supplemental Fig. 3) was enhanced compared to Fc-wt.
STAP takes as input a set of sequences and their measured binding intensities to TFexp, a set of TF motifs (including TFexp) and learns TF-binding models that can be used to predict binding affinity of any new sequence.
While lacking of HS or GAGs on cell surface, relative hRNase3 and HBPRNase3 32 41) binding intensities to both CHO-pgsD677 and CHO-pgsA745 cells dropped about 50%.
(iv) GeF-seq data suggest that protein-binding signal intensities to the genome should correlate with protein-binding affinities to the cognate target sequences, but this needs to be shown experimentally.
The differential binding intensity of the Eσx to the promoters of 1,139 genes, whose transcription is directly affected by σ, is shown in Figure 3a.
These technologies synthesize a large set of DNA sequences and measure the binding intensity of the TF to each of those sequences.
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