The results thus far demonstrate that multiple non-binding influences on ChIP-seq signals can be identified and quantified.
Higher predictability, of course, means that the total non-binding influences on the signal are greater, and it suggests that a smaller portion of the signal reflects what is truly of biological interest (binding for TFs and modifications for HMs).
In order to facilitate high-throughput virtual screening, these scoring functions often treat subtle influences on binding energy like conformational entropy and solvation energy only superficially [ 27, 53], thus sacrificing accuracy for the sake of greater speed.
In TM3, His219Ala, Leu220Ala and Leu222Ala have influences on αCGRP binding and cAMP production; they are likely to indirectly influence the binding site for αCGRP as well as having an involvement in signal transduction.
We show that type II binding can have a profound influence on binding affinity for CYP3A4, and the difference in binding affinity can be as high as 1200-fold.
The analysis sheds light on RanBP3 with regard to its weak Ran-binding activity, its influence on the cargo-binding selectivity of CRM1, and its ability to stabilize a CRM1/Ran/NES complex in the nucleus.
Botly, L.C.P. * & De Rosa, E. (2007) Cholinergic influences on feature binding.
This suggests that the formation of stable homodimers itself leads to significant basal allosteric influences on ligand binding kinetics.
Our results show that incubation media used for the endotoxin binding assay have an influence on the binding capacity of clay minerals.
Characterizing ligand binding and its influence on the detailed supra-fold dynamics of multi-domain proteins therefore requires atomic resolution techniques.
A special focus was studying the role of the remaining truncated version of CREI with regard to the transcriptional regulation of target gene expression, the ability of DNA binding, and the influence on the chromatin structure.
