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However, due to the limited number of IDRs that fold upon protein binding in this test set, it is difficult to make statistically sound conclusions about differences in method performance.
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Agona strains were highly consistent in Calcofluor binding in our test systems.
Furthermore, in addition to the bioMérieux 4th generation combo test (DuoULTRA) and antigen-only test (HIV p24 II), the Siemens ADVIA CHIV also had poor sensitivity for pBV43-D, pBV60-D and pBV42-12BF, indicating binding of antigen around position 170 in this test.
One possibility for the weak binding in our tests is that additional proteins that were not present in the gel shift reaction mixture are required for maximal complex formation.
By contrast, Elvitegravir induced little or no change in the Tm of Mos1 Tnp, suggesting there is no binding in the conditions tested.
The positive data sets are constructed from 226 known binding sites in our test data set by splitting the known binding sites into single- and double-strand binding sets according to the binding preference of each TF.
For each verified binding site in our test set, we randomly select 20 genomic locations (from the same promoter sequence) with the average binding site of length 12, which results in a background set that is 20 times larger than the test set.
When the researchers used a chemical trick to simulate ubiquitin binding in the globular section, tests showed that this binding was necessary to activate OTUB1 to inhibit chain formation.
For all the known binding sites in, we compute counts and which are the number of times and, respectively, where is the width of the verified binding site in the test set and is the threshold specified by quantile.
The assay involves binding IgE in the test specimen to an anti-IgE, which has been immobilised on a solid-phase matrix.
Statistical Analysis (a one-way ANOVA) was performed to evaluate significant decrease in EV binding in presence of monosaccharide tested.
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