The finding, in GST pulldown assays (Fig. 1C), that WW2 3 cannot mediate binding to SGK when other WW-domains are present may be an artifact due to a sequence in the longer multiple WW-domain constructs being unable to fold correctly or due to occlusion of the binding site in this context preventing WW-domains 2 and 3 from binding to SGK.
This paper contributes to understandings of the production of normativity, i.e. the ways in which actors come to see rules as binding, in the context of corporate reporting regimes.
In addition, we propose a novel method, which uses Miyazawa and Jernigan protein protein potential measurements, for assessing molecular binding in the context of immune complexes.
Taken together, the results from these immunoprecipitation experiments indicate that the KE is not accessible to antibody binding in the context of intact viral particles.
To examine the role of Dab2-sulfatide binding in the context of thrombin-mediated platelet activation, N-PTB was pre-incubated with sulfatide liposomes and protein-lipid complexes were treated with thrombin.
There are many uncertainties when using whole protein as an antigen, such as localizing the antibody binding sites, directing the antibody response to certain sites and defining how amino-acid changes alter antibody binding in the context of a complex structure.
Fdx binding in the context of other IscS binding partners, namely IscU and bacterial frataxin, has also been explored.
More likely, TBC1D15 and TBC1D17 use LC3/GABARAP binding in the context of Rab GAP activity to orient growing isolation membranes to the surface of the cargo.
In addition, interaction between Hox proteins and these cofactors differentially affects the binding preferences of Hox proteins enabling the emergence of "latent specificity" of Hox binding in the context of the Hox-cofactor complex [ 9].
While quinate and shikimate complexes were pursued with the objective of clarifying the noncovalent basis of substrate and product binding, it may be more instructive to view protomer A binding in the context of analyzing the carbinolamine intermediates.
