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However, cdc13 -1 cells that also carry either the tlc1 Δ48 or yku80 -135i mutation are more temperature sensitive than cdc13 -1 cells, suggesting that capping is compromised further by reduced Est2p telomere binding in these backgrounds (Vega et al. 2007).
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This precaution was taken to avoid the selection of peptides binding in the background.
In principle, this can be done to create optically enhanceable donors only when species are bound, thereby potentially enabling the measurement of binding constants in high-background environments.
Since most background binding in MHC multimer analysis leads to signals in either one or multiple (three or more) channels (Hadrup, unpublished observation), background is reduced approximately tenfold by this gating strategy.
These results suggest that there are lots of nonspecific and background binding in the highly ranked peaks.
Background binding in the presence of 1 mM GRGDS peptide was subtracted to obtain specific binding.
Consequently, there is a need for other macrophage tracers with lower background binding in peri-articular tissues.
Analysis of human Ep-CAM-negative CHO cells revealed background binding in the order of 10 sites/cell (data not shown), indicating that the MDA-MB-231 cell line can be considered as Ep-CAM negative.
Background BMP binding in the absence of rhPln.247 was subtracted.
These studies reveal the issues associated with BODIPY-based probes, including narrow Stokes shifts causing potential interference from Raman and Rayleigh scattering, and high lipophilicity leading to nonspecific binding and high background in lipid membranes.
The chemical potential depends on the number of transcription factors present in the cell, on the binding probability to background sites elsewhere in the genome (which have a sequence similar to the target sequence by chance), and on the functional sites in the in the genome other than the binding site in question that may compete for the same protein.
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