In response to heat shock, PARP-1 PARylates PAP, which decreases its binding to the transcribed gene and results in the inhibition of polyadenylation and subsequent alteration of gene expression.
To test if Rab6A interferes with KIF1C motor domain binding to microtubules, in vitro transcribed and translated, radiolabelled, full-length KIF1C was tested for microtubule binding in the presence and absence of purified Rab6A using a microtubule co-sedimentation assay.
By doing so, the ward doctor was supported by the pharmacist in the transcribing process.
Several genes with putative chemosensory functions, such as odorant binding proteins (OBPs), odorant receptors, antennal carrier protein, arrestins, gustatory receptors, and pheromone binding proteins, were transcribed in the A. gambiae salivary gland (3 were highly expressed, 4 were moderately expressed, and 19 were weakly expressed; see Additional file 1).
To overcome these limitations, we have developed a rapid and large-scale approach to characterize binding of in vitro transcribed labeled RNA to ~9,400 human recombinant proteins spotted on protein microarrays.
While there is no detectable FnrL binding site in the intergenic region between divergently transcribed hemB and rcc01809 genes, there is a ChIP-seq peak with an FnrL recognition sequence located within rcc01809.
The kinetic properties of the enzymes as well as the binding of TPP to the in vitro transcribed RNA of the proposed operons suggest that the vitamin B1 homeostasis in S. aureus is strongly regulated at transcriptional as well as enzymatic levels.
Lastly, H3.3 is accumulated on transcription factor binding sites in the regulatory regions of most genes as well as on the coding regions of transcribed genes in cultured mouse ES cells [ 6], and in cultured human HeLa cells [ 17].
Uncapped RNA used for in vitro binding assays was in vitro transcribed using the MEGAScript kit (Ambion) per manufacturer's instructions.
