A similar approach was used to investigate lesion binding in the RB69 gpolymeraserase where the Y567A and L561A mutations were more amenable to structural studies involving Gh or syn OG.
Two distinct CRISPR types, distinguished by the repeat sequence, are found in both the Syn OS-A and Syn OS-B' genomes.
To assess whether this increase in promoter activity was specific to the MTF-1 binding site or an indirect effect of MTF-1 over-expression, we abolished the MRE binding site in β-Syn −1.1/−0.6 kb by site-directed mutagenesis.
The FL α-syn antibody recognized monomeric α-syn in the α-syn tg mice (Figure S1A).
Meanwhile, in the absence of the negative charged lipid DOPS, we did not observe any inhibition in lipid mixing at various α-Syn concentrations within experimental errors, even though there is some binding of the α-Syn to VAMP2 containing v-vesicles (Supplementary Figure 1c).
Docking of the analogues to the modeled binding sites furnishes explanations, in structural terms, for exclusive recognition of the syn conformer despite the non-homologous design of the binding sites.
He had previously played in a group called the Syn, which also featured the future Yes guitarist Peter Banks.
Though less toxic than N-SYN, the SYN in itself is toxic, and induces nigrastriatal degeneration when overloaded.
Syn-attack is disfavored because of the steric interaction between two aromatic moieties in the TS syn.
No significant alterations in DAT binding were seen in the 1∶10 A53T α-syn or in either concentration of GFP.
