Furthermore, we have shown (Fig. 2A) that all three tested ADAMTS13 antibodies do not bind to VWF and therefore the increase in binding in the presence of VWF results only from specific binding to ADAMTS13.
Isothermal titration calorimetry (ITC) experiments on aspartate binding in the presence of eight different sodium concentrations showed that GltTk indeed binds three sodium ions per aspartate molecule, the same number as GltPh (Fig. 2).
a, PvRBP2b(161–1454) binding in the presence or absence of anti-PvRBP2b monoclonal antibodies (3E9, 4F7, 6H1, 8G7 and 10B12) or anti-PfRh4 monoclonal antibodies (10C9), analysed by flow cytometry.
"We found that there was decreased HDAC3 binding in the presence of this Rett mutation, and there was also decreased binding of the transcription factor, FOXO3, and this corresponded with decreased gene expression," says Nott.
The residual fraction of FL-p53 binding in the presence of overexpressed Δ133p53 to the BAX and PUMA promoters (approximately 20% of the binding in its absence) was larger than that to the miR-34a and p21WAF1 promoters (9% and undetectable, respectively).
For thrombin, smaller negative ΔCp values are observed for ligand binding in the presence of sodium ions compared to the other alkali ions, probably due to stabilising effects on the protein or changes in the bound water structure.
(D), (H) Autoradiograms showing full displacement of 125I-[Tyr3]-neurotensin 125I-[Tyr3]-neurotensin 125I-[Tyr3]-neurotensin
Non-specific binding in the presence of 10 μM PMPA was lower than 0.5% for all compounds investigated.
The total cellular activity was corrected for non-specific binding in the presence of 100 μM AMD3100.
Displacement of specific [125I]-AB-MECA binding in the presence of different A3R antagonists revealed estimates for the K i values as detailed in Table 1.
(B), (F) Autoradiograms showing total binding of 125I-[Tyr3]-neurotensin 125I-[Tyr3]-neurotensin 125I-[Tyr3]-neurotensininding (in the presence of 100 nM neurotensin).
