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These observations are consistent with a model in which inhibitors bind in one subunit of COX-2 and inhibit 2-AG binding in the other subunit of the homodimeric protein.
Thus, the critical residues in the Man5A CBMs are either not essential for substrate binding in the other members of this family or the two CBMs are evolutionarily distinct from the members available in the current protein database.
Intriguingly, the 2.1 Å density map of bovine CIII clearly shows that it contains two opposite orientations of the full-length UQCRFS1N peptide (1 78 amino acids), with the N terminal binding in one CIII protomer and the C terminal binding in the other CIII protomer (Fig. 1C E).
In contrast, clomipramine had no effect on I-MIBG binding in the other ileal carcinoids (case nos. 1, 3 and 4).
There is no reason to assume that protein binding in the other patients was likely to be significantly different between the two groups; in addition, albumin levels in groups 1 and 2 were similar.
Although CR10 would have been the appropriate choice for the DxxxD motif, the absence of a tryptophan residue from this domain, which we used as a non-perturbing endogenous reporter for ligand binding in the other domains, made this an unsuitable choice.
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The effect of putrescine binding and conformational changes in the loop provide a possible mechanism by which changes in one putrescine binding site are relayed through the dimer interface to the putrescine binding site in the other monomer.
Generation of Friedel's salt is the dominant chloride binding process in the other cement pastes, as shown in Fig. 5.
X-ray crystallography demonstrated that this scaffold bound in three different binding modes, depending on the substituents; two of these were in the pterin binding site, whilst in the other binding mode, the ligand occupied an area of the active site perpendicular to the canonical binding site.
We are unable to find any Staf-binding sequence in the other two fish MRP-RNAs.
Further, our data showed that p85α can bind PTEN, promote PTEN activity and membrane binding in the absence of other proteins.
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