Sentence examples for binding in the major from inspiring English sources

These are (1) binding in the major groove, (2) binding in the minor groove, and (3) intercalation between nucleotide bases.

These include conventional triplex binding in the major groove of the DNA double helix and triplex invasion in which two preferably antiparallel PNA oligomers invade and open this DNA double helix through formation of an internal PNA DNA PNA Watson Crick Hoogsteen triplex.

On the one hand, DNA recognition is provided by a rigid bicyclic peptide scaffold derived from triostin A that contains nucleobases to allow for binding in the major groove of dsDNA.

This construct placed two positively charged amines adjacent to the major groove edges of two symmetry-related guanines, providing a model for probing how cation binding in the major groove modulates the structure and stability of DNA.

In order to determine the site of the opposite end of the sub-genomic fragment, we performed restriction mapping using an enzyme that cleaves in the mtDNA major arc (Fig.  3C, PmeI) in combination with a series of enzymes that cut in the mtDNA major and minor arc (Fig.  3C, BclI, MfeI, BglI, HindIII, NheI, SacII, AflII and XhoI) and a radioactive probe binding in the major arc.

A more detailed look at region 1 reveals the greatest disparity between Rep2 and Rep5 occurs at the RBE binding interface in the major groove of the ITR (Fig. 6F).

The Pho4 homodimer contacts its binding sequence in the major groove on two opposite faces of the DNA, with the helix-loop-helix domain on one face and the remainder of the protein, including its activation domain, on the other face of the DNA [34].

It is demonstrated that disruption of a cation binding site in the major groove by modification of the N7-position on the purines, which is the major site for DNA alkylation, is enthalpically destabilizing.

Because N7-alkylG readily depurinates to an abasic site, c7G (see Figure 3 for structures), which is stable, was introduced into DNA to determine how elimination of the cation binding site in the major groove would affect DNA stability and structure.

Overall, the distance restraints derived from these data help to define better the structure of SLVI and to allow the localization of five well-defined divalent metal-binding sites in the major groove of the RNA.

Structural superposition and subsequent mutagenesis experiments suggest that the basic protrusion motif plays a role in direct binding to the major groove in RNA/DNA hybrid, as well as in establishing the co-ordination among modules in RT necessary for proper function.