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At these physiological metal concentrations, the concentration of Mn+2 is not large enough to shift the equilibrium of binding in the direction of metal-phosphate complex formation.
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These structures show the peptides binding in the plus direction, that is, opposite that of their wild-type counterparts.
Actinin-4 has one actin-binding domain at the N-terminus, and actinin-4 monomers can form a homodimer by binding in the opposite direction to form a dumbbell-shaped structure (Otey and Carpen, 2004).
Using EMSA, we detected evidence of allelic differences in protein binding in the same direction as our predicted imbalance at 4 of the 7 sites (Fig. 2d), for a total of 6 of 9 supported imbalances.
However, dynein prefers to rotate to the right, suggesting that with every step along a microtubule, it binds to the closest available binding site in the forward direction.
Dynein has a net preference to move along a right-handed helical path, suggesting that the heads tend to bind to the closest tubulin binding site in the forward direction when taking sideways steps.
These results show that NES binding in the minus vs plus direction is determined by placement of the ΦXΦ pattern at the N- or C-terminal end of the NES peptide.
In particular, the interactions of Arg and Lys with the binding sites ensured the occurrence of binding and the direction of the N-terminal of the peptide.
The largest enzyme classes tend to be oxidoreductases or subunits of oxidoreductases, and the relationships among members of these groups point in the direction of shared binding capacities accounting for the sequence relatedness, e.g. Fe-S clusters.
Perfusion by means of the portal vein (anterograde direction) produced pimonidazole binding in the pericentral region of liver similar to that observed for pimonidazole binding in vivo.
Our results differed from the initial hypothesis also in the direction of the difference in SERT binding.
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