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Because subsequent work raised questions about the existence of these calcium-binding sites, we assayed calcium binding in solution to the InlB N-cap.
As a control to assess metal binding in solution to the proposed site involving D210, N212, and H309, we also prepared and analyzed D210A APE1.
The DSC experiments described above demonstrated that the thermostability of SitA was most notably increased by Mn2+ and Zn2+ ions, and that upon addition of EDTA the binding in solution to Mn2+ was reversible, while binding to Zn2+ was not appreciably reversible.
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The chapter presents a fractal analysis for the binding of estrogen receptors in solution to different ligands immobilized on a SPR biosensor chip surface.
Because many DNA binding drugs exhibit large changes in absorbance or fluorescence on binding, these changes are commonly used to determine the distribution of free and bound drug in solution to construct binding isotherms that may be used to obtain binding constants.
For example, the binding of 40 nM ERα+17β-estradiol in solution to 300 RU of TIF2 immobilized on a SPR biosensor surface [J. Clin. Ligand Assay 18 (4) (1995) 215] requires a dual-fractal model.
Thus, the analysis of the binding and dissociation of beta amyloid (Aμ) in solution to fibril surfaces is discussed.
It is known that FGF2 also binds to soluble heparin and this binding in solution can compete with the binding of FGF2 to HSGAG.
Finally, to prove the binding of A38 to VACV with both binding partners in solution, corresponding to in vivo conditions, Fluorescence Correlation Spectroscopy (FCS) was performed (Fig. 3c).
Compactin treatment relocalized N-ras mutant/RBD complexes to the cytoplasm, allowing us to assess binding in solution.
Compactin treatment relocalized K-ras mutant/RBD complexes to the cytoplasm, allowing us to assess binding in solution.
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