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One possibility is that these SNPs are disrupting TF binding in regions of open chromatin.
Of these approximately 200 SNPs, 36 were predicted to influence TF binding, in regions of sequence conservation of over 70% in human-mouse; the alignments for two such regions are indicated in additional files [See Additional file 2].
While the study is interesting, it is based on a very small sample, and it is not obvious that FDDNP binding in regions of the brain that show tau deposition at autopsy in NFL players necessarily implies tau deposition in this study as FDDNP is not specific for tauopathies.
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As there is a bias towards Ft phosphorylation increasing binding, more intermediate binding occurs in regions of high Fj even though less favourable Ds (light purple) is available.
An interpretation of RF binding at pattern 3 DMRs with respect to CpG-density is that RF binding occurs in regions of elevated CpG-density, with coordinated methylation changes in adjacent lower CpG-density regions.
Computationally found binding sites are predominantly located in regions of experimentally proven bindings and these regions are also considerably well conserved among analyzed species.
(A ) Ethylene treatment results in an increase of EIN3 binding in three regions of the EBF2 promoter, corresponding to an increase in steady-state mRNA levels.
An enrichment of accessible chromatin and TFBS binding in conserved regions of the first intron is consistent with their role in active gene regulation.
For instance, in people with Parkinson's disease, use of placebos has been shown to increase dopamine binding in certain regions of the brain.
After tissue dissection and sample preparation, the radioligand binding in brain regions of interest was quantified by scintillation spectroscopy.
Purified RNA was quantified with a nanodrop apparatus and tested for genomic DNA contamination based on a PCR (Phusion) protocol using a primer pair binding in nontranscribed regions of the X-chromosome (Primer code: X-1435; sequence available on request).
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