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For determination of [H]PiB binding in homogenates of Alzheimer's disease brains, 1 nM [H]PiB (American Radiolabeled Chemicals, St . Louis MO; specific activity 72.4 Ci/mmol) was incubated with 100 µg tissue in 1 ml PBS.
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In a previous study [25], [3H]flumazenil binding in brain homogenates of ABP and B6 mice was measured after exposure to a novel situation.
Troglitazone also significantly decreased the activity of 3β-hydroxysteroid dehydrogenase in homogenates of human ovarian tissue.
Expression of IDO1mRNA was measured in whole brain, lung and liver homogenates and in homogenates of 4 5 isolated Peyer's patches (PPs).
The concentration of the carbonyl in LV homogenates was standardized on the protein unit (nmol carbonyl group/mg protein) in homogenates of LV.
Highly aggregated molecules were apparent in homogenates of daf-2 RNAi-treated worms, but not in homogenates of vehicle- or NT219-treated animals (Fig. 4D).
There was a very low specific binding in cortical tissue homogenates of control brains.
Table 1 presents the literature data of Kd and Bmax for each tracer and target along with the values of Vu,brain determined in rat brain slices or calculated from reported data of binding in brain homogenate (fu,brain).
The HIF-1α-DNA binding activity was highly increased in indomethacin alone group, which was significantly decreased in gastric homogenates of pantoprazole treatment group, signifying pantoprazole significantly relieved hypoxic condition induced by indomethacin.
TNF-α, CXCL-1, CCL-2, and MPO were detected in lung homogenates of the BLV group (Fig. 2).
The level of S-nitrosylation was greater in lung homogenates of female mice as compared to lung homogenates from male animals.
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