As we now know, the amount of specific binding in brain of 11C- R -PK11195 is quite small, BP ND = 0.8 [11C- R -PK11195 greater variability and the need for much larger sample sizes to detect statistically significant differences.
NF-(B mediates its action by binding DNA, therefore to examine whether the increase in p52 is associated with a change in the composition of the DNA bound NF- B dimer, gel shift assay was used. As previously reported with other tissue [ 28- 30], a general increase in NF-(B DNA binding is evident in brains of old mice compared to young (Fig. Supplemental S3C).
This phenomenon was even more pronounced at 60 min post injection, where the isoflurane anesthetized mice still had brain concentrations close to 10%ID/g, whereas no discernible [18F]flumazenil binding remained in brain of the awake mice.
After tissue dissection and sample preparation, the radioligand binding in brain regions of interest was quantified by scintillation spectroscopy.
In a previous study [25], [3H]flumazenil binding in brain homogenates of ABP and B6 mice was measured after exposure to a novel situation.
Ex vivo studies (with increased sensitivity as compared to in vivo μPET) analysing the binding capacity of [C]PIB in brains of 15-month-old PS1/APP mice did not show any significant difference either.
In this study, we screened affected transcription factors using a new strategy in which alterations in their DNA binding were comprehensively analyzed in brains of a commonly used HD mouse model (R6/2) which expresses an N-terminal (exon1) fragment of mutant huntingtin (mutant Nhtt).
The amyloid ligand H-PIB was also used to quantify brain amyloid levels, and the profiles for H-PIB autoradiography binding in the brains of 6- to 24-month-old APPswe mice and 8- to 24-month-old wt mice are displayed in Fig. 4a.
In addition, PPAR-binding protein (Pparbp), which was downregulated in brains of KIKO mice and fibroblasts from FRDA patients (13), was also downregulated.
Figure 3 Quantitation of [ 18 F]flutemetamol binding in the brain of mouse models of AD.
