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The presence of specific binding in a reference region may underestimate the calculated specific binding.
The assumption of no specific binding in a reference region is not valid for all tracers, and especially not for non-selective OR ligands.
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For example the text states that fucose is critical for cholera toxin binding, in reference to an experiment where a fucose inhibitor causes a 2-fold reduction in binding activity.
Kerry told the Financial Times on Wednesday that any agreement in Paris, where world leaders meet in three weeks' time, is "definitively not going to be a treaty" and there were "not going to be legally binding reduction targets like Kyoto", in a reference to the world's only previous binding climate treaty.
(of a reference to a binding) being a reference that reads the value of the binding.
In practice, the binding site(s) of the candidate ligand can be judged based on its competitive binding against a reference ligand but can not be optimized.
In order to normalize for inter-strain differences we performed our calculations using SRTM, although there is a small displaceable binding in both reference regions analysed in our study.
Violations of model assumptions such as activation-induced changes in regional blood flow or specific binding in the reference tissue have negligible effects on lp-ntPET performance.
Assumption 3 (on the specific binding in the reference tissue, k 3r): Specific binding of radioligand is negligible in the reference tissue (k 3r = 0).
Yet, it should be mentioned that both plasma input-derived DVR 1 and SRTM-based BPND estimates will be negatively biased by the presence of specific binding in the reference region (pons), as discussed below.
Non-specific binding in the reference cell was subtracted.
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CEO of Professional Science Editing for Scientists @ prosciediting.com