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Using rational design, several amino acids involved in substrate binding have been identified and site-directed mutations have revealed that residue H115 plays an important role in binding.
Moreover, from the contour maps, the key features vital to ligand binding have been identified, which are important for us to trace the important properties and gain insight into the potential mechanisms of intermolecular interactions between the ligand and receptor.
Recently, using a PCR-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure two consensus sequences for HMGA2 binding have been identified.
In mammals, important amino acid motifs involved in receptor binding have been identified and mutation studies reveal the importance of the N-terminus [ 20].
The residues of CXCL8 responsible for GAG binding have been identified by site-directed mutagenesis and NMR experiments to include Arg, Lys, Lys and Arg that are contained in the C-terminal α-helix as well as His and Lys contained within the proximal loop [ 9].
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Although β-alanine binding has been identified throughout the hippocampus, limbic structures, and neocortex, unique β-alaninergic neurons with no GABAergic properties remain unidentified, and it is impossible to discriminate between β-alaninergic and GABAergic properties in the CNS.
The DNA sequence required for LANA binding has been identified as the LBS.
The full length protein was shown to bind erythrocytes in a culture supernatant based erythrocyte binding assay [31] and the domain (region II) responsible for mediating the binding has been identified and characterized [8].
Intriguingly, no functional activity for this binding has been identified to date.
This domain contains homology with the C-type lectin, but to date no distinct carbohydrate binding has been identified.
The C-terminal DNA binding domain in SusR has no significant similarity to DNA binding domains of other known TFs; however, a putative helix-turn-helix motif presumably involved in DNA binding has been identified near its carboxy terminus [ 12].
More suggestions(17)
obligations have been identified
settings have been identified
binding have been associated
binding have been attributed
binding have been calculated
binding have been studied
binding have been characterized
binding have been defined
binding have been found
binding have been suppressed
binding have been shown
binding have been developed
binding have been written
binding have been performed
binding have been considered
binding have been mapped
binding have been investigated
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