Thus a tag on the N-terminus is preferable, as was also found for tagged ACT88F actin in Drosophila (43), although we cannot rule out that it will interfere with myosin binding, since myosin has been predicted to interact with the N-terminus of actin (45).
Because the CD22 molecule is rapidly internalized after antibody binding (unlike CD20), it has been predicted that anti-CD22 would be an excellent vehicle for the delivery of toxic moieties to B cells.
Structure activity relationships of 33 derivatives of 5- 5-Phenyl-furan-2-ylmethylene -2-thioxo-thiazolidin-4-one have been studied and binding mode of this chemical class has been predicted.
It has been predicted that binding of cyclic-di-GMP causes a conformational change to allow UDP-Glc access to the catalytic site [ 32 ], but very recent structural studies have provided insights into the mechanism [ 35].
The relevance of the Pol α CTD structure might extend to other polymerases such as REV3, the catalytic subunit of the translesion polymerase ζ and archaeal DNA polymerases for which the existence of zinc-binding motifs at their C-terminus has been predicted, based on the presence of a conserved pattern of cysteine residues (Cann et al, 1998; Shen et al, 2004; Gan et al, 2008).
Then, using the same simulation protocol, the binding mode of salacinol and its derivatives has been predicted.
In contrast it has been predicted that Escherichia coli K-12 contains 314 regulatory DNA-binding proteins [56].
AP-2 and ZF5 binding sites have been predicted together in liver by Smith et al [ 43].
Additional putative FoxO-target genes and their potential cis-regulatory binding sites have been predicted by systematic bioinformatic approaches [ 11].
While no p53-binding sites have been predicted in the miR-15b promoter [30], it is possible that p53 down-regulates miR-15b expression through an indirect way.
