Several mutations in the RIG-I regulatory domain that affected RNA binding have been characterized [6], [7], [14].
Thanks to a recently designed pressurization cell using krypton at high pressure, a new gas binding site has been characterized that reveals an alternate pathway for gaseous ligands.
A high affinity binding site has been characterized using radioligands in U937 monocyte membranes, where a novel radiolabelled EET agonist bound in a specific, saturable and reversible manner, resulting in the production of cAMP production with similar potency as 11,12-EET 11,12-EET-EET.
Another mRNA binding protein that has been characterized is PfIRPa, an iron regulatory-like protein; it binds to an iron-responsive element, a stem-loop structure formed at 5' and 3' UTRs of mRNA, and functions to inhibit translation or to modulate the stability of mRNA [ 15].
One of these proteins, the CUG triplet repeat binding protein (CUGBP1), has been characterized in detail.
For example, the newt HH ribozyme, which is embedded in the Satellite 2 family (sat2), has been associated with a ribonucleoprotein (RNP) in the ovaries [ 45]; an RNA binding protein (NORA) has been characterized as a promising candidate as a constituent of this complex [ 19].
The myeloid-specific nuclear transcription factor CCAAT/enhancer binding protein epsilon (CEBPE) has been characterized previously [8].
The genome binding profile of RBf1 has been characterized in both Drosophila embryos and larvae, and both studies revealed that RBf1 interacts with numerous genes related to cellular signaling pathways, in addition to previously characterized cell cycle genes (Acharya et al. 2012; Korenjak et al. 2012).
The glycan receptor binding preference of H2N2 viruses has been characterized using solid phase binding assays [24] and more recently using crystal structures [25], [26].
