Lastly, a QSAR study for the binding affinity to the corticosteroid-binding globulin using Cramer's steroid dataset is carried out.
Sex hormone binding globulin was measured using the Spectria SHBG IRMA kit (No. 68562; Orion Diagnostica, Espoo, Finland).
To estimate the circulating fraction of free testosterone, we measured the serum level of sex hormone-binding globulin (SHBG) using an immunoassay (Diagnostic Products Corp ,San Francisco, CA, USA), and SHBG was expressed in nanomoles per liter.
We evaluated the percentage changes in estrone, estradiol, free estradiol, testosterone, free testosterone, androstenedione, dehydroepiandrosterone sulfate and sex hormone-binding globulin (SHBG) using generalized estimating equations.
We measured concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and sex hormone-binding globulin (SHBG) using a time-resolved fluoroimmunoassay (Delfia; Wallac, Turku, Finland).
Total serum 17β-estradiol was determined by competitive immunoenzymatic colorimetric assay (NovaTec Immunodiagnostica GmbH, Dietzenbach, Germany) and serum sex hormone binding globulin (SHBG) levels using solid-phase, chemiluminescent immunometric assay (Immulite® 1000 SHBG, DPC [Diagnostic Products Corporation], Los Angeles, USA).
A small molecule, such as testosterone, may be separated from large components, such as sex hormone binding globulin (SHBG) and the testosterone/SHBG complex, by using dialysis membranes with an appropriate cutoff for molecular size.
Sex hormone binding globulin (SHBG) was measured using an enzyme-linked immunosorbent assay using commercially available kits (DRG Diagnostics, Marburg, Germany).
Sex hormone binding globulin (SHBG) was assayed using a fully automated system (Immulite; DPC, Inc., Los Angeles, CA, USA) at the Reproductive Endocrinology Laboratory at Massachusetts General Hospital, using a solid-phase two-site chemiluminescent enzyme immunometric assay.
Because thyroid binding globulin may impede the reliability of FT4 assays, it is preferable to use circulating total thyroxine as a measure of thyroid gland activity in pregnant women, because thyroid-binding globulin concentrations are elevated during pregnancy (Stagnaro-Green et al. 2011).
