The binding function of the Hc domain is essential for BoNTs to bind the neuronal cell membrane, therefore, removal of the Hc domain results in a product that retains the endopeptidase activity of the LC but is non-toxic.
Because helix-4 mutations disrupt the F-actin binding function of the FABD, these results show that F-actin binding per se is not required for the inhibition of the NLS function.
In addition, these data provide insight into the less characterized cys-domain and suggest an important functional role for a less conserved region of the cys-domain that is distinct from the RNA binding function of the highly conserved cysteine-rich motif.
Further, the antibodies were chosen such that the antibody binding does not interfere with receptor binding function of the hemagglutinin on the virus.
Rather, the suspected RNA binding function of the C-terminal domain of yeast mtLeuRS (Labouesse, 1990) suggests that it functions by stabilizing the mutant tRNAs.
Structural and proteomic analysis of PIH-N reveals that substrates of the R2TP complex are designated by phosphorylation and recognized by the phosphopeptide binding function of the PIH1D1 subunit.
Thus, we suggest that substrates are marked for assembly by the R2TP complex through phosphorylation of a short, highly acidic motif by CK2 (or other acidophilic kinases), which is then recognized by the phosphopeptide binding function of the PIH1D1 subunit.
Anyway our observations strongly suggest that for ERs, the DNA binding function of the receptor as well as its interaction with co-regulators have been conserved due to selective pressure.
In order to investigate whether the methylhistone H3 binding function of the G9a ANK domain is required for mESC differentiation and the accompanying de novo DNA methylation of key pluripotency genes, G9a-null mESCs (knock-out; KO) were used to generate stable clonal cell lines expressing either wild-type (+WT) G9a or G9a containing various ANK domain point mutations mentioned above.
