DAVID classification of proteins by molecular function revealed that most of the proteins were involved in binding (including binding with iron ion, collagen, heme, sugar, and tetrapyrrole) or had structural activity or antioxidant activity.
A global GO analysis [ 70] of unisequences in relation to molecular function revealed that the largest number of transcripts was related to protein binding, followed by peptidase and transferase activities, calcium-binding, enzyme regulation, nucleic acid binding, structural protein and transporter activities, lipid binding, and transcriptional regulation.
GO mapping to molecular function revealed that a majority of genes from the tissue transcriptomes of wild silkmoths have almost equal distribution for 'binding' function (property of binding macro-molecules) and catalytic activity.
MM-PBSA calculations revealed that binding was energetically favorable and driven by the electrostatic interactions.
Solvent mapping revealed that TPP interacted with binding hot spots within the PPARγ ligand binding domain.
However, a closer look at the binding site revealed that the mutation displaced the side-chains that form the PLP-binding surface (Fig. 3B).
Molecular function assignment revealed that oxidoreductase activity, transferase activity, ion binding and nucleic acid/nucleotide binding accounted for the largest portion of the P. mariana unique genes identified (~47% combined).
