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"Religions have a strong binding function and a cohesive element," de Waal says.
The sequence of Unc45b suggest that it can be divided into at least three homology regions: three TPR motifs on the N-terminus, a C-terminal ∼420 residues UCS domain that has a myosin binding function, and a large central region of uncertain function that includes regions of limited homology to β-catenin (armadillo repeats).
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HSP90 consists of four structural domains: a highly conserved N-terminal domain (NTD) with ATPase activity; a middle domain (MD) with client protein binding functions; and a C-terminal domain responsible for the interaction with cofactors (dimerizing domain) [ 11– 13].
Here we develop a coarse-grained physics-based model of protein folding and binding function, and compare it to a popular informational model.
Regions which between them have several overlapping functions: in the juxtamembrane region; a nuclear localisation signal, ezrin/radixin/moesin protein, rapsyn and ERK MAP Kinase binding function, and at the C terminus a regulatory tyrosine governing WW, SH2 and SH3 domain interactions.
These genes have a nucleic acid binding function and are associated with pyrophosphatase GTPase, transferase and catalytic activity.
Numerically, the top five categories are metabolism, unclassified, transcription, protein with binding function, and protein fate.
The first shows closest similarity to polynucleotidyl transferase, Ribonuclease H fold (IPR012337), a domain associated with nucleic acid binding functions and found in a variety of proteins including HIV RNase H, transposases, and exonucleases [ 79, 80] (Fig. 6).
Transcript isoforms that show male-specific expression include: trehalase (FBtr0071537), which encodes an enzyme for trehalose metabolism; another B-box affiliate (FBtr0086572), which encodes a product with protein binding functions; and Vha55, which encodes a vacuolar H+ ATPase (FBtr0301661) [ 43].
Of the 13 genes with transport and binding functions in Cluster A, ten ORFs (ftn, so1580, so2045, alcA- so3031- so3033, so3063, so3063, viuA, so4743) were shown in an earlier study to be induced in chromate-challenged wild-type MR-1 cells compared to untreated cells [ 11, 13].
The opposite trend of increasing transcript abundance from Phase I to Phase III was observed for functional groups that included transcription (11), protein synthesis (12), protein fate (14), protein with binding function (16), and to a lesser extent with interaction with cellular environment (34).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com