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The non-binding fractions and the binding fractions were pooled together separately and assessed for inhibitory activity on OPC differentiation using the in vitro substrate assay outlined below.
The pooled binding fractions were further concentrated and the buffer exchanged to a buffer composed of 250 mM 6-aminocaproic acid, 25 mM Bis Tris, pH 7.0 using Amicon ultra centrifugal filter devices.
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A high binding fraction of 86.2% was found, which seems in good agreement with the difference of bacterial susceptibility tests observed in broth and serum.
S, F, W and B denote the starting sample, flow-through fraction, wash fraction and binding fraction, respectively.
Among viral and bacterial proteoms, the binding fraction of peptides is even higher.
BSA was used as a negative control and MAPF (microtuble binding fraction) containing MAP2A, MAP2B, MAP1 and tau as a positive control, where MAP2 constitutes 70% of MAPF.
The results displayed in Fig. 7 present both the binding fraction and the induced calcium rise obtained as a function of ρL.
A log-log plot (included in supporting Material S1) rules out a polynomial dependence of the binding fraction on the illumination power.
sOB-R, formed by cleaving the ectodomain of membrane-anchored leptin receptors, represents the major leptin binding fraction in blood [37].
BSA was used as a negative control and the microtubule binding fraction (MAPF) provided by the cytoskeleton assay kit containing MAP2A, MAP2B, MAP1 and tau as a positive control, where MAP2 constitutes 70% of MAPF.
To analyse whether the contribution of motif- and context-binding residues to the binding energy in the globular domain differs, we computed the "context binding fraction" for each domain position, that is, the ratio of context contacts vs. all interchain (i.e. motif plus context) contacts observed for this residue.
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