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To aid in the design of new biosensors based on mitochondria (MT), we have investigated the feasibility of detecting ion fluxes through the MT-membrane K+-ion channels using piezosensors with MTs immobilized either by hydrogen bonding or thin polypyrrole (PPy) binding film.
Semiquantitative EDS analyses, as transects across the film, and compositional mapping of the aggregates indicate that the binding film consists of an amorphous phase rich in S and Fe and minor P, with variable amounts of K, Ca, Na, and Mg, depending on the particle to which they adhere.
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The vehicle, usually a resin dissolved in a solvent, dries to a tough film, binding the pigment to the surface.
At a surface pressure of 30 mN/m no protein fluorescence was detected, consistent with protein exclusion from the interface or less SP-A binding to the film.
They would likely suffer from lack of signal in cases where a molecular layer binding NPs to film does not promote the formation of an immobilized high-density, close-packed NP network (e.g., a monolayer of biomolecules designed to bind 1 NP per molecule) due to the lack of coupled resonant dipoles excitable by s-polarized illumination at a 0° illumination angle.
Scolamacchia et al. (2005) interpreted the nature of the S-Fe-rich film binding the particles to the precipitation of an amorphous phase from either S-rich solutions or condensing hygroscopic fluids, derived from the contemporaneous ejection of magmatic, hydrothermal fluids and phreatic water during several phases of the eruption.
The heparin binding to tau films was investigated in the presence of [Fe CN 6]3−/4− or benzoquinone redox probes.
The electrochemical behaviors of the films binding to FPBA and the competition with saccharide were investigated by cyclic voltammetry.
It is shown that the amount of CF2- and CF3-bindings in the film is the dominant correlation factor between XPS and surface tension.
At the same time the combination formulation has, because of the magnesium component, a 50% higher phosphate-binding capacity per film tablet than pure calcium carbonate (39.1 mg vs. 19.5 mg) [14].
However, as a consequence of its magnesium component, OsvaRen® has a 30% higher phosphate-binding capacity per film tablets compared to pure calcium acetate (39.1 mg vs. 29.7 mg).
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CEO of Professional Science Editing for Scientists @ prosciediting.com