This point was studied in detail in the case of the decidual prolactin gene, dPrl, where modifications after insertion provided multiple transcription factor binding sites that bind factors that interact with each other [ 206].
To identify the binding factors that may interact with CR4.2, we first used MatInspector in Genomatix Suite (München, Germany) (Quandt et al., 1995; Werner, 2000; Cartharius et al., 2005) to search for potential trans-acting factor binding sites on CR4.2.
The cognate DNA-binding transcription factors that interact with these promoter-proximal and -distal DNA sequence elements have been identified [4], [6].
Hypoxia-inducible factors are DNA-binding transcription factors that interact with specific nuclear cofactors under hypoxia, and they transactivate a series of hypoxia-associated genes to trigger adaptive responses.
A subset of the DNA-binding transcription factors that interact with Groucho/TLE proteins in the haematopoietic compartment, including Hes1, Runx-1 and Pax5, have been shown to play a role in regulating TLE phosphorylation and modulating its activity [ 49, 50].
An important feature of homotypic site clustering is that it facilitates cooperative binding of factors that interacts to moderate or weaker sites [ 24].
Rather, the meiotic recombinational activity is determined combinatorially by the binding of multiple transcription factors that interact with each other [ 135].
Eukaryotic genes are transcriptionally regulated by the coordinated interaction of multiple protein factors that interact with discrete binding sites and with each other [ 20].
It is also unresolved why the position of the 5′ Hε is critical for both RNA packaging and translational repression, although it could be related to cap binding factors that require a short enough distance to interact with and regulate the HP Hε complex for both activities.
The regulation of gene expression in mammalian cells is controlled by approximately 200 transcription factors that interact with distinct DNA binding sites (1).
