CUG repeats have the ability to form RNA hairpins that are accumulated into ribonuclear foci (Taneja et al., 1995) and sequester a number of RNA-binding factors, including the alternative splicing regulators of the Muscleblind family MBNL1-MBNL3 (Millet et al., 2000; Fardaei et al., 2002).
With recent data and less stringent criteria we detected 81 CCAAT binding factors including those TFs previously detected.
These new regions are characterized by higher local enrichment of macroH2A and lack of H3K27me3, but significant overlap with other marks and binding factors, including CTCF.
In this study, we report in silico sequence analysis of this 150-bp enhancer and identification of its multiple binding factors, including AP1, MEF2, NFAT, Runx1 and TBX5.
It is also known that the promoter region of the iNOS gene contains a multiplicity of consensus sequences for the binding of transcription factors, including the HRE, thus rendering the iNOS gene hypoxia- inducible (Melillo et al, 1995).
In silico analyses predicted a change in the binding of several transcription factors including the introduction of a Sp1-like binding site caused by the introduction of the -151 variant.
In vivo binding depends on several factors including the cellular environment and the chromatin state of the bound region.
In general, the degree of p53 binding depends on various factors including the state of the p53 protein, its cofactors, and the sequence composition of the p53-RE [ 5, 32].
The possibility also exists that other calcium- and phosphate-binding factors, including non-proteinaceous components, may be present in the serum in large amounts and that these factors may remain soluble even in the presence of excess calcium or phosphate.
By contrast, our direct measurements of differential levels of histone acetylation allowed us to select an experimentally informed set of candidate regulatory regions that showed differential distribution of the binding sites for 3 of the 4 differentially expressed factors including the binding site for Gata2, the potential relevance of which we went on to validate experimentally.
