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Although currently available bioinformatics methods cannot detect all transcriptional regulatory elements, our thorough analysis of OR promoters shows that in the case of this gene family, experimental approaches have probably already identified all the binding factors common to large fractions of OR promoters.
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In addition, there are factors specific to each bubble as well as factors common to all.
Algorithms in the Genomatix program were used to identify transcription factor binding sites common to the regulatory sequences in the 5′ regions of co-regulated set of crystallin and other genes as compared to a set of control genes.
In Figure 2B, we also highlight several conserved transcription factor binding sites common to each sequence, including sites for CRX, THR, and AP-1.
Our comprehensive expression data set allowed us for the first time to try such an in silico approach and we compared the promoters of genes co-expressed in the poorly characterized glycogen trophoblast and sinusoidal TGC populations as examples, identifying several modules of putative transcription factor binding sites common to the promoters of each group.
In each specific tissue cluster, 1kb upstream sequences from PA cluster start site were scanned for finding transcription factor (TF) binding sites common to at least 60% of input sequences by MatInspector in Genomatix Genome Analyzer.
Furthermore, known transcription factor binding sites (TFBS) common to most of the fish G-expressing promoters, might enable the future design of fully synthetic or hybrid promoters with improved efficacy of VHSV G-protein expression in epithelial fish cells.
It is believed that there is a core of transcription factor binding sites (TFBS) common to all PVs, with additional individual differences, although most of the available information focuses only on a handful of viruses.
As shown in Table 2 after grouping all the binding sites belonging to the same class of transcription factor, only four binding sites are common to all the genomic fragments active in the sensory vesicle (white background).
An iron-binding protein common to many external secretions.
Summary: Current methods for motif discovery from chromatin immunoprecipitation followed by sequencing (ChIP-seq) data often identify non-targeted transcription factor (TF) motifs, and are even further limited when peak sequences are similar due to common ancestry rather than common binding factors.
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