To provide support that b-nisin binds to lipid II associated with germinated spores, competition binding experiments were performed.
Saturation binding experiments were performed on PC-3 cells at 4 °C.
To determine the inhibitory mechanisms, additional radioligand binding experiments were performed.
In vitro binding experiments were performed as previously described [9] with 20-μm brain cryosections from transgenic APP23 (N = 2), Tg2576 (N = 2) and APPswe-PS1dE9 (N = 2) mice and wild-type control mice (N = 5) that were sacrificed after in vivo studies were finalized (Table 2).
To investigate the possibility that E-cadherin may interact with integrins expressed on non-leukocytic cells, cell adhesion and solid-phase receptor ligand binding experiments were performed using a pentameric E-cadherin construct designed to detect low affinity, high avidity interactions.
Ribosome binding experiments were performed as previously described [24].
Displacement binding experiments were performed with a constant [125I]α-BTX concentration of 2.0 nM.
Saturation binding experiments were performed with [125I]α-BTX concentrations ranging from 0.03 100 nM.
In vitro GST fusion protein binding experiments were performed as described [30].
The binding experiments were performed in the absence or presence of Hfq.
The Dab2 N-PTB binding experiments were performed in 10 mM Tris-HCl (pH 7.4) and 100 mM NaCl.
